Isoelectric focusing of Bacteroides melaninogenicus group beta-lactamases
نویسندگان
چکیده
منابع مشابه
Beta-lactamases: determination of their isoelectric points.
Important discrepancies in isoelectric points (pI) of beta-lactamases were observed depending on the experimental procedure used for their determination: isoelectric focusing (IEF) in sucrose density gradients or analytical IEF in thin-layer polyacrylamide gels (PA). The variations, negligable in the case of TEM-like beta-lactamases, appeared to be important in the case of cephalosporinases and...
متن کاملCharacterization of bacteroides melaninogenicus.
Fifty-eight human isolates of Bacteroides melaninogenicus, 42 from a variety of clinical infections and the rest from normal flora, were studied for pigment production and ultraviolet light fluorescence and by forty biochemical and other tests, including end-product analysis by gas-liquid chromatography. In a number of instances, tests were repeated several times and the results were reproducib...
متن کاملLipids of Bacteroides melaninogenicus.
The lipids of Bacteroides melaninogenicus were readily extractable with chloroform-methanol. Three per cent of the fatty acids were not extractable. The neutral lipids contained 4% of the extractable fatty acids, the stench characteristic of these organisms, and 0.5 mumole of vitamin K(2) isoprenologues K(2)-35, K(2)-40, and K(2)-45 per g (dry weight). This is one-fifth to one-tenth of the vita...
متن کاملNotes on bacteroides melaninogenicus.
element is still in doubt. Efforts are being directed toward the interpretation of the structures which can be visualized in bacteria (DeLamater, Ann. Rev. Microbiol, 8, 23, 1954). The description and (lemonstration of the nuclear configurations found in normal vegetative cells of S. typhosa form the basis for comparison with cells treated with colchicine described by Minsavage and DeLamater (J...
متن کاملMolecular hybridization versus isoelectric focusing to determine TEM-type beta-lactamases in gram-negative bacteria.
Isoelectric focusing and molecular hybridization with a TEM DNA probe were used to screen for TEM beta-lactamase in 328 bacterial isolates representing 11 gram-negative genera. The TEM enzyme was detected in 50% of isolates, and nine additional types of beta-lactamase could be identified in 36.9% of isolates. The TEM gene was detected in 53.6% of isolates. The results obtained by both methods w...
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ژورنال
عنوان ژورنال: Antimicrobial Agents and Chemotherapy
سال: 1981
ISSN: 0066-4804,1098-6596
DOI: 10.1128/aac.19.5.700